Neutrophil chemotaxis in Wegeners granulomatosis and idiopathic pulmonary fibrosis
نویسندگان
چکیده
Background: The presence of anti-neutrophil cytoplasmic antibodies (ANCA) in Wegeners granulomatosis (WG), implicate the neutrophil as a key effector cell. Previous studies have reported elevated neutrophil counts in the lung although the determinants of neutrophil chemotaxis in the WG lung are unknown. Methods: BALF cell counts, myeloperoxidase (MPO) and chemokines were measured in 27 patients with WG, 20 disease controls with idiopathic pulmonary fibrosis (IPF) and 6 healthy controls. CXCL-8, IL-1β, ENA-78, G-CSF and GM-CSF were measured by ELISA. The neutrophil chemotactic potential of BALF was investigated using the under agarose method and specific antibodies examined the role of CXCL-8 and IL-1β. Results: WG BALF had an increased neutrophil percentage and elevated MPO, CXCL-8 and G-CSF concentrations compared with healthy controls. Chemotaxis of control neutrophils towards BALF from patients with active (p=0.006) and remission WG (p=0.077) and IPF patients (p=0.001) was increased compared with normal controls. BALF induced chemotaxis correlated with BALF IL-1ß (r=0.761,p=0.001) and CXCL-8 (r=0.640,p=0.012) in WG and was inhibited by anti-CXCL-8 (85%, p<0.001) and antiIL-1β (69%, p<0.001). Conclusions: Our study confirms a neutrophilia and pro-inflammatory alveolar milieu that persists in clinical remission. CXCL-8 and IL-1β appear to play important roles in the neutrophil chemotactic response to BALF.
منابع مشابه
Otologic Manifestations and Progression in Patients with Wegener’s granulomatosis: A Survey in 55 Patients
Introduction: Granulomatosis with polyangiitis (GPA; also known as Wegener’s granulomatosis) is a primary systemic vasculitis involving the ear, nose and throat system (ENT) and lower respiratory tract. Because of the lack of knowledge regarding the clinical findings of GPA due to the limited number of studies, the current study was designed to investigate the prevalence and nature of the otolo...
متن کاملidiopathic pulmonary arterial hypertension
cytometry analysis of BAL cells. Table 1 integrates information given in our published paper, clearly demonstrating that the number of BAL neutrophils was fair in our case series. This unfortunately prevented a definitive evaluation of whether polymorph nucleates represent a source of IL-17. Nonetheless, as shown in figure 1, in selected cases with a significant number of BAL neutrophils (two s...
متن کاملidiopathic pulmonary arterial hypertension sildenafil
cytometry analysis of BAL cells. Table 1 integrates information given in our published paper, clearly demonstrating that the number of BAL neutrophils was fair in our case series. This unfortunately prevented a definitive evaluation of whether polymorph nucleates represent a source of IL-17. Nonetheless, as shown in figure 1, in selected cases with a significant number of BAL neutrophils (two s...
متن کاملGene Polymorhisms of the Interleukin-10, Angiotensin-Converting Ensyme and Transforming Growth Factor-β1 Genes in Granulomatous Disorders, Pulmonary Sarcoidosis and Wegeners Granulomatosis
متن کامل
Bilateral Facial Paralysis and Otitis Media as the First Presentations of Wegener’s Granulomatosis: A Case Report
Introduction: Cranial nerve palsy in Wegener’s granulomatosis is a curious incident, particularly if it occurs without kidney or lung involvement. In a review of medical articles, only 1 case of Wegener’s granulomatosis with bilateral facial nerve palsy was found. Case Presentation: The patient was a 16-year-old female who presented with pain and hearing ...
متن کامل